Jordan Montenegro

Montenegro’s Medium (MM)

Serum-free, low-cost axenic medium enabling robust Naegleria fowleri growth.

Figures

Growth curve in MM

Growth in Montenegro’s Medium from 0–168 hours
Fig. 2 — Growth in MM (0–168 h).

Fresh vs old MM

Fresh vs old Montenegro’s Medium at 48 and 72 hours
Fig. 3 — Fresh vs old MM at 48 h and 72 h.

Passage timing & method

Passage timing TD3–TD5 and comparison of DT vs CT
Fig. 4 — Passage timing & method (TD3–TD5; DT vs CT).

DAY 0

Micrograph day-0
day 0

DAY 3

Micrograph day-3
day 3

DAY 7

Micrograph day-7
day 7

DAY 10

Micrograph day-10
day 10